Sunday, December 15, 2013

DNA Reptile. (Replication)

DNA replication is a process where DNA replicates itself.
There are many enzymes that help in this process.

Helicase- The scissors in the group.
Starting from the Origin of Replication (the starting point) Helicase, (In class we used a yellow triangle to represent Helicase. You can do this if you need some visual aids) breaks down the Hydrogen bonds that connect the two bases.
It does this to unwind the double helix, and prepare a single stranded DNA replication.

Then RNA primase comes on and places rna pairings to the single stranded dna. For example, if you had a strand that read CTAG, the RNA paired with it would be, GAUC, with the Thymine being replaced with Uracil. 
It does this to allow DNA Polymerse III the ability to read the strands, because it places rna which has a 3' OH base, which DNA polymerse III can read. 

DNA polymerse III then comes in and reads the strand of RNA and creates the complementary nucleotides. Each new base forms phosphodeister bonds. 

DNA Polymerse I then replaces the rna with the newly created bases. 

Ligase glues all the okazaki fragments together. 


No comments:

Post a Comment